Shocking facts about HIV test kits
Have you read the package insert notes from HIV test?
If not, then maybe you might be surprised. The HIV/AIDS industry is a big scam.
The HIV tests prove nothing.
The HIV/AIDS dissease is caused by a fake diagnose, fear mongering and toxic HIV-medications, vaccines, antibiotics, party drugs and more.
There is no HIV virus. In Africa and poor countries people die of starvation and not enough nutrion and clean water.
Text: Carl Grinde Date: 30 August 2011
Text also shared from linked pages
Uppdated April 8 2013
(To find words in a text quickly, press CTRL F on a PC. Tip: copy some text and paste in the text search field)
Ok guys I took the HIV test, again
It has felt a little strange to do hiv tests the last times and I doubt that I will do it another time. Even if I question the reality of hiv, I must say that all nurses on the clinics have been very nice and helpful.
I feel liberated from the lie of HIV/AIDS and it feels good. At the same time that invisible hand rules our world, we can call the it the C.I.A.
or George Bush & Co.
They work in silence and can probably go in and manipulate medical records or even our thoughts and opinions through microchip implants straight into the brain.
Check out these movies about the CIA and the dark forces that governs our world.
Is it "just a movie"?
The Adjustment Bureau
Eyes wide shut
about secret societies
The Good Shepherd
One Mainframe To Rule Them All
America: Freedom to Fascism
Mind Control - America's Secret War
The Manchurian Candidate
Manchurian candidate Documentary
MK ultra documentary
Although this was also Hollywood movies, ask yourself: is there a hidden hand that rules the world or not? Smart medicines
is on the rise. Of course they have conducted research on human brains, probably on prisoners, probably many who died, with mass electrodes and components inside the body. How could they otherwise just show up that kind of advanced inventions outright that works on people directly? artificial eyes,
artificial implants in the ear,
clearly they have done research on humans.
I will pay for speaking out about this
I know I will pay a big price for speaking out about this and September 11th, deadly vaccines,
the rothschild's world domination, Freemason's
hidden occult identity
Since I did an HIV test is my DNA somewhere where I do not have control. CIA or Illuminati
can steal it and accuse me
for a crime that I didn't commit.
It's not only about HIV/AIDS
The global elite does more evil things.
Other big lies are September 11th,
more about September 11th,
. The corporate deception about HIV/AIDS
is not alone. They own all media networks so they can do whatever they want.
About the HIV tests
Italian version French version Greec version
HIV-1 Western Blot Kit Orasure Epitope Inc
6. Do not use this kit as the sole basis of diagnosis of HIV-1
7. A Negative result does not exclude the possibility of HIV-1 infection.
Roche The COBAS AmpliPrep TaqMan HIV-1 Test is not intended for use as a screening test for the presence of HIV-1 in blood
Roche The COBAS AmpliScreen HIV-1 Test, version 1.5
This test is not intended for use as an aid in diagnosis.
Roche The AMPLICOR HIV-1 MONITOR Test, v1.5
is not intended to be used as a screening test for HIV-1
The AMPLICOR HIV-1 MONITOR Test, v1.5 is not intended to be used as a screening test for HIV-1 or as a diagnostic test to confirm the presence of HIV-1 infection.
Roche UltraSensitive Roche Monitor Test, v1.5- Boom Extraction
This test is not intended to be used as a screening test for HIV or as a diagnostic test to confirm the presence of HIV infection.
There are six diagnostic methods currently in use to detect HIV infection: EIA for antibody detection, Western Blot for the detection of antibody directed against specific viral components, viral culture, EIA for HIV-1 antigen detection, indirect immunofluorescence assay, and radio immunoprecipitaion assay. Most of these methods, however, do not provide a sensitive and specific method for the direct measurement of HIV-1 viral activity.
The NucliSens HIV-1 QT assay is not intended to be used as a screening test for HIV-1
The NucliSens HIV-1 QT assay is not intended to be used as a screening test for HIV-1 nor is it to be used as a diagnostic test to confirm the presence of HIV-1 infection.
The OraSure HIV-l Western Blot Kit is not intended for use with blood
The OraSure HIV-l Western Blot Kit is not intended for use with blood, serum/plasma or urine specimens, or for screening or reinstating potential blood donors.
ABBOT HIV test HIVAB HIV-1/HIV-2 (rDNA) EIA
HIVAB HIV-1/HIV-2 (rDNA) EIA is an in vitro enzyme immunoassay for the qualitative
detection of antibodies to human immunodeficiency viruses type 1 and/or type 2
(HIV-1/HIV-2) in human serum, plasma, or cadaveric serum.
Sensitivity and Specificity
At present, there is no recognized standard for establishing the presence or absence of
antibodies to HIV-1 and HIV-2 in human blood.
Sensitivity for HIV-1 antibodies was computed based on the clinical diagnosis of AIDS. For
HIV-2, sensitivity was expressed in terms of detection rate using investigational confirmation
assay results as a basis for comparison.
Specificity is based on assay of blood donations from random donors. Sensitivity for HIV-1
antibody was shown to be equivalent to a previously licensed test based on comparative
studies in various clinical groups including AIDS, ARC, and High Risk.
WARNING: FDA has licensed this test kit for use with human serum, plasma, or
cadaveric serum specimens only. Use of this licensed test kit with specimens
other than those specifically approved for use with this test kit may result in
inaccurate test results.
BioLytical Laboratories the INSTI Kit, INSTI HIV-1/HIV-2 Rapid Antibody Test
bioLytical Laboratories develops and manufactures a rapid HIV diagnostic product, the INSTI™ Kit. It is a unique, simple-to-use diagnostic kit used in the diagnosis of HIV infection. At 60 seconds for a reading, INSTI Kit is the fastest HIV diagnostic assay in the world today.
BioLytical Laboratories Insti HIV-1/HIV-2 antibody test
Absence of antibodies to HIV does not indicate that an individual is absolutely free of HIV-1 or HIV-2; HIV has been isolated from seronegative
individuals prior to seroconversion.
BioLytical Laboratories Insti HIV-1/HIV-2 antibody test
The INSTI HIV-1/HIV-2 assay has not been validated for detection of antibodies to HIV-1 Group O or N
subtypes. (According to the AIDS/HIV industry HIV viruses are now divided into different subtypes. By stating that the INSTI test has not been validated for detection of antibodies to HIV-1 Group O or N
subtypes, they also tell you that their test is not a way to make sure if you are "HIV positive" or not!)
There is no single standard for detecting the sensitivity or specificity of an antibody test for HIV in human sera, plasma or whole blood. However,
the generally accepted method to express the sensitivity and specificity of a given test in terms of the
detection rate is to compare results to approved supplemental assay results, such as ELISA and Western Blot.
BioLytical Laboratories INSTI HIV-1 Antibody Test Kit
..for use in testing the effectiveness of INSTI HIV-1 Antibody Test Kit.
(Ok so this stuff is only for testing the test kit. Cool.)
INTENDED USE The controls provided in this kit are intended to be used as part of the user’s quality assurance program.
Ortho Clinical Diagnosis Inc Trypanosoma cruzi (T. cruzi) ORTHO T. cruzi ELISA Test System
This assay is not intended for use as an aid in diagnosis.
Intended Use: Added the use of cadaveric (non-heart-beating) specimens.
GENELABS DIAGNOSTICS HIV-1 BLOT 1.3 WESTERN BLOT ASSAY
FOR RESEARCH USE ONLY NOT FOR USE IN DIAGNOSTIC PROCEDURES
Bio rad GS HIV-1/HIV-2 PLUS O EIA
First "real" HIV test i found
GS HIV-1/HIV-2 PLUS O EIA 32588
480 tests for detection of antibodies to human immunodeficiency virus types 1 and 2 (HIV-1 and HIV-2) in human serum, plasma, and cadaveric serum
Bio rad HIV test insert note -the antibody sandwich technique
Human immunodeficiency virus types 1 and 2 recombinant and synthetic peptides GS HIV-1/HIV-2 PLUS O EIA Biological Principles of the Procedure
The Genetic Systems HIV-1/HIV-2 plus O EIA is an immunoassay based on the principle of direct the antibody sandwich technique. Microwell strip plates (the solid phase) are coated with purified antigens:
gp160 and p24 recombinant proteins derived from HIV-1;..
(So the surface is coated with antigens and proteins derived from HIV-1. They have covered the surface with HIV virus components and if your blood has anti-bodies to this components then you have HIV in your blood.
Hm..I wonder how they manage to harvest all these HIV components. And how do they make it stay on the microwell strip plate? Is it small HIV viruses laying on these strip plates waiting and doing just fine without any blood or T-Cells? Is a microwell strip plate really a good environment for a HIV virus to live in?
I thought the HIV virus needed a host cell.
How do they purify them, the "antigens"? And how do they harvest them so they can put it on the microwell strip plate? Is it dead?)
How do they know that it is only HIV particles that sticks to the microwell strip plate surface and not some other stuff? I mean measuring HIV seems to be so hard anyway. These HIV-test clearly shows that finding HIV-viruses in blood is not easy. They need to re-test with other standards to make sure that it really IS HIV or not.
So how do they collect all the "purified antigens gp160 and p24 recombinant proteins derived from HIV-1"?
Are they using HIV-test in their cultivating tank to make sure it really is HIV before they put it on the microwell strip plates?
Therefore, the GS HIV-1/HIV-2 Plus O EIA had an estimated specificity in this study of 99,92% (24,228/24,247; 95% confidence interval: 99,88-99,96)
Lots of references to tests with Western Blot. But Western Blot can give indeterminate results. Hm..(..?)
All HIV test Kits have the words "In vitro use" what does "In Vitro use" mean?
What does it mean when a pregnancy test says "For in-vitro diagnostics use only"?
Best Answer - Chosen by Voters
in vitro means and artificial environment outside an organism. So basically, it means for testing outside your body, not inside. I guess you will have to pee on a stick or something.
Ok guys, below is the heavy section and the hot potatoe: Biotechnology Papers, Protein Engineering, Design & Selection
Humanization strategies for an anti-idiotypic antibody mimicking HIV-1 gp41.
(..seriously what is this?)
Characterization of "regulatory" idiotope-specific T cell clones to a monoclonal anti-idiotypic antibody mimicking a tumor-associated antigen (TAA).
(..man this is advanced shit!)
(What names will the comming HIV gene theraphy products/tests have? ..Mimicry-Dummy Virus test? I.D.I.O.T.I.C and ..IDIOT-TEST?)
Y Saeki, J J Chen, L F Shi, S Raychaudhuri, H Köhler in The Journal of Immunology (1989)
OK! But now we PROMISE! This HIV test is ..a good one. For Sure.
Orgenics.com with "Know for sure" HIV test kits
Merck ELISA/Western blot tests for HIV
A positive result on the ELISA screening test does not necessarily mean that the person has HIV infection. There are certain conditions that may lead to a false positive result, such as Lyme disease, syphilis, and lupus.
A positive ELISA test is always followed by a Western blot test. A positive Western blot confirms an HIV infection. A negative Western blot test means the ELISA test was a false positive test. The Western blot test can also be “indeterminate,” in which case additional testing is done to clarify the situation.
(My question: If A positive ELISA test always is followed by a Western blot test and a positive Western blot confirms an HIV infection, why not do the western blot test directly and skip the ELISA test? Oh ..I forgot, the Western blot test can be “indeterminate”)
However, nonspecific reactivity on
Western blots can occur with HIV-negative sera, leading to
inconclusive or indeterminate results. The causes of indeterminate
results, although not clearly understood, have been
associated with contaminating cellular antigens on viral lysatebased
blot strips, autoantibodies, pregnancy, elevated bilirubin
levels, and in vitro hemolysis (4).
ELISA stands for enzyme-linked immunoassay. It is a commonly used laboratory test to detect antibodies in the blood.
(hm.. possible HIV antibodies is not specificly mentioned here)
An antibody is a type of protein. The body's immune system produces antibodies when it detects harmful substances, called antigens. Examples of antigens include microorganisms (such as as bacteria, fungi, parasites, and viruses) and chemicals.
Antibodies are also be produced when the immune system mistakenly considers healthy tissue a harmful substance. See: Autoimmune disorders
Each type of antibody is unique and defends the body against one specific type of antigen.
Viral Transcription HIV Antibodies -for sale at www.immuquest.com
Why is there no images of the HIV anti bodies, the "human proteine against HIV"?
Maybe because there is no HIV virus and no HIV-anti bodies?
HIV1 integrase - Mouse Anti-HIV1 integrase
Integrase is an enzyme, a product of the HIV1 Gag/Pol gene; the other two enzymes being reverse transcriptase and protease. The human immunodeficiency virus (HIV-1) uses an enzyme, a so-called integrase, to carry out the integration of its viral DNA into the host chromosome thereby tricking the host cell machinery into making viral proteins. The HIV-1 integrase is a protein of 32 kDa and is composed of three domains. No cellular homologue of HIV integrase has been described, so potential inhibitors to this enzyme could be relatively nontoxic.
HIV1 P17 Gag - Mouse Anti-HIV1 P17 Gag
HIV1 p17 is the matrix protein of the Gag polyprotein which performs highly complex orchestrated tasks during the assembly, budding, maturation, and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA
Wantai HIV (1+2) Ab ELISA Wantai HIV (1+2) + HIV-1 p24 ELISA
The Sensitivity is not always 100 %. Besides it's being used in Chineese bloodbanks and we know how that dictator government works and where the blood comes from right?
Complete list of approved HIV test and Manufacturers
"HIV/AIDS" FDA approved products Time line 2000-2010
Suggested websites for further information:
Why the “HIV Tests” Can’t Tell You Whether You Have HIV
Where is the electron microscopic data?
Causes of a False Positive HIV Test
On the average, 0.2 percent of ELISA tests give a false positive HIV test result that are then proven incorrect by a Western Blot test, notes Goaskalice.com, the Columbia University student health website. Autoimmune diseases, recent vaccinations, multiple pregnancies or prior infections with hepatitis B, malaria or tuberculosis can stimulate the production of antibodies that cross create with HIV antigens, thus producing a false positive HIV test.
HIV test are not intended to be used as a screening test for HIV
In fact, viral load tests carry disclaimers stating they are not intended to be used as a screening test for HIV or as a diagnostic to confirm the presence of HIV infection (Roche Amplicor viral load test).
Why isn’t an antibody test that’s verified by another antibody test good enough to say someone is infected with HIV?
Why not use the test kit to diagnose an HIV infection?
I think I know why they write that they don´t want their test kits to be used to diagnose an HIV infection.
It's because they know that there isn't any HIV virus.
Why people get sick and die is because there is a silent war on our society.
Investigate the vaccine programs in Africa, chemtrails, narcotics
and toxic medicines.
HIV test accuracy? Ask the manufacturers
Why the “HIV Tests” Can’t Tell You Whether You Have HIV
TEST KIT MANUFACTURERS ADMIT THEIR "HIV TESTS" ARE UNRELIABLE
Reported Causes of False-positive EIA and Immunoblot Tests for HIV Antibody*
Cross-reactive antibodies to some human leukocyte antigens (HLA) that
develop as the result of prior blood transfusion or during pregnancy
Auto-antibodies found in some rheumatologic diseases
Heating of serum
Freeze-thaw of serum
Receipt of gamma-globulin
Recent vaccination (influenza, hepatitis)
Some liver diseases
Positive RPR tests (syphilis, some autoimmune diseases)
Technical errors in testing
Hiv Tests Are The Biggest Scam!!!
HIV tests exposed
Did You Know That HIV Tests Come Up Positive For HIV Test Components?
If HIV does kill T-cells, how come they have an immortal T-cell line from which they extract all their HIV for the test kits?
"At present there is no recognized standard for establishing the presence or absence of
HIV-1 antibody in human blood." (Abbott Laboratories, ELISA HIV Antibody Test Insert,
section "Sensitivity and
"At present there is no recognized standard for establishing the presence or absence of HIV-1 antibody in
human blood." (Abbott Laboratories, ELISA HIV Antibody Test Insert, section "Sensitivity and
"EIA testing cannot be used to diagnose AIDS... The risk of an asymptomatic person with a repeatedly
reactive serum developing AIDS or an AIDS-related condition is not known." (Abbott Laboratories, ELISA
HIV Antibody Test Insert, section "Limitations of the Procedure")
"Clinical studies continue to clarify and refine the interpretation and medical significance of the presence
of antibodies to HIV-1." (Abbott Laboratories, ELSA HIV Antibody Test Insert, section "Limitations of the
Western Blot Test
"Do not use this kit as the sole basis of diagnosis of HIV-1 infection." (Eptope, Inc., Western Blot HIV
Antibody Test Insert, section "Limitations of the Procedure")
“The clinical implications of antibodies to HIV-1 in an asymptomatic person are not known.” (Calypte,
Cambridge Biotech HIV-1 Western Blot Kit, section “Limitations of the Serum and Plasma Procedure”)
PCR "Viral Load" Test
"The AMPLICOR HIV-1 MONITOR test, is not intended to be used as a screening test for HIV or as a
diagnostic test to confirm the presence of HIV infection." (Roche, Amplicor HIV-1 Monitor Test Kit,
section "Intended Use")
Ortho-Clinical Diagnostics, Inc
more of their HIV test and products
0,5 procent of all humans are immune to the HIV virus
..eh really? So what is this HIV/AIDS deadly virus all about?
Typical "End AIDs" page
They are trying to rebrand AIDS to mean something else than the virus.
The campaign includes fight against poverty. It is truly an important thing to do.
But since AIDS is mixed with other issues it gets hard for people to tell one thing from another.
It gets harder to tell the fraud from the help needed to the poor people.
Prejudice is also one issue they want to be included in the AIDS issue.
"Housing for everyone" is also a headline. Hm that would be perfect but lets be realistic.
Housing problems and poverty, prejudice are real serious stuff but it's not the same thing as AIDS.
"Lead the way" ..with mobile testing vans
This campaign promotes a system with testing teams that will talk to people in the streets and ask them to do an HIV test.
The staff will be sending out mobile testing vans and having people go door-to-door in randomly selected census tracts in Hillcrest and North Park to offer on-the-spot tests.
Read MARK CONLAN's article about the HIV testing campaign: Big Brother Is Testing You.
Resistance is fruitful
It's OK to question AIDS
Advanced Biotechnologies You want a virus? We produce them!
ABI's expertise are HIV, HTLV, HSV, VZV, EBV, CMV, HHV-6, HHV-7, HHV-8, RSV, SIV ...
Product Range: Antibodies, Cell Biology Products, Infectious Disease Antigens, Test Kits. Viral Proteins.
A supposed photo of HIV virus
Why is the images of the virus so small? The blue dots is the HIV virus i guess but I would like to se a little more detailed and closer photo to be convinced of the truth in this photo.
Lennart Nilsson is a very good photographer but I'm not sure if he has much knowledge about viruses. Maybe they will have him to photograph the E hec and the Ebola viruses as well?
HIV not guilty
A homepage that don't like people questioning HIV and AIDS
This page that call people like me a liar is totally anonymous.
No face on the people behind the homepage.
No public info about who the registrar of its webadress.
Not a telephone number. Only an email adress.
Step out in the light or shut up. What you say has no importance.
"For more information about HIV denialism and for the real facts about HIV and AIDS, please go to www.AIDStruth.org.
email@example.com" (..yeah right)
AIDS research pdf magazine the virus illustration looks like an orange with some clove spice in it with the color changed to blue instead of brown.
(From Merck liars manual) HIV-1 originated in West-Central Africa, chimpanzee infected humans. HIV-1 originated in West-Central Africa in the first half of the 20th century when a closely related chimpanzee virus first infected humans.
The illustration of a HIV virus fits exactly inside a hexagon which is one important symbols of the illuminati.
Why only computer graphics of the virus? Why not a photo?
hivbook.com illustration of HIV virus with illuminati symbolism
Why not a photo of the virus? The shape of the virus uses the same symbolism as in the pdf from Wantai. It's just graphics, drawings.
Bristol-Myers Squibb HIV drug
REYATAZ (atazanavir sulfate)
Symptoms: blisters, mouth sores, swelling of your face, potentially death.
Bristol-Myers Squibb REYATAZ (atazanavir sulfate) HIV drug
"..symptoms that could be serious and potentially cause death"
REYATAZ (atazanavir sulfate) HIV "medicine" side effects, symptoms, or conditions, including the following:
Mild rash (redness and itching) without other symptoms sometimes occurs in patients taking REYATAZ® (atazanavir sulfate), most often in the first few weeks after the medicine is started, and usually goes away within 2 weeks with no change in treatment.
Severe rash may develop with other symptoms that could be serious and potentially cause death.
If you develop a rash with any of the following symptoms, stop using REYATAZ and call your healthcare provider
Shortness of breath
General ill-feeling or "flu-like" symptoms
Muscle or joint aches
Conjunctivitis (red or inflamed eyes, like "pink-eye")
Swelling of your face
Yellowing of the skin and/or eyes may occur due to increases in bilirubin levels in the blood (bilirubin is made by the liver).
A change in the way your heart beats may occur. You may feel dizzy or lightheaded. These could be symptoms of a heart problem.
Diabetes and high blood sugar may occur in patients taking protease inhibitor medicines like REYATAZ. Some patients may need changes in their diabetes medicine.
If you have liver disease, including hepatitis B or C, it may get worse when you take anti-HIV medicines like REYATAZ® (atazanavir sulfate).
Kidney stones have been reported in patients taking REYATAZ. Signs or symptoms of kidney stones include pain in your side, blood in your urine, and pain when you urinate.
Some patients with hemophilia have increased bleeding problems with protease inhibitor medicines like REYATAZ.
Changes in body fat have been seen in some patients taking anti-HIV medicines. The cause and long-term effects are not known at this time.
Immune reconstitution syndrome has been seen in some patients with advanced HIV infection (AIDS) and a history of opportunistic infection. Signs
and symptoms of inflammation from previous infections may occur soon after starting anti-HIV treatment, including REYATAZ.
Gallbladder disorders (including gallstones and gallbladder inflammation) have been reported in patients taking REYATAZ.
Other common side effects of REYATAZ taken with other anti-HIV medicines include: nausea; headache; stomach pain; vomiting; diarrhea; depression; fever; dizziness; trouble sleeping; numbness, tingling, or burning of hands or feet; and muscle pain.
"..symptoms that could be serious and potentially cause death"
Bristol Mers Squibb HIV drug Sustiva
See the rising sun symbol in their logo. The rising sun is Freemasonry symbolism
for global power and the "beginning for their new world"
She had "Aids" for 12 years, skipped the drugs and then was recovered
HIV tests are the biggest scam!!!
Mark Conlan's Extended Interview
He is an AIDS/HIV rethinker and does not believe in the official AIDS/HIV theory
Mark Conlan's Magazine Zengers
alternative views on AIDS/HIV
The biggest problem with “Lead the Way” is that it’s based on lies. Some of them are the standard lies the AIDS establishment has been putting out for years, including the basis of the test itself. The standard test does not measure live, infectious virus; instead, it tests for antibody reactions to the nine proteins that, together, are presumed to make up HIV
Heal San Diego
alternative views on AIDS/HIV
Myth: AIDS is a disease of the immune system.
Truth: AIDS is a disease of toxicity
Myth: "HIV tests" detect antibodies to HIV.
Truth: "HIV tests" react to cellular particles found in all of us.
Myth: "HIV drugs" prolong life..
Truth: "HIV drugs" cause AIDS, and fatal organ failure.
reverse transcriptase HIV enzyme bullshit
Health Education AIDS Liaison, Toronto
The Other Side of AIDS
What's on the surface of an HIV-test?
Microwell strip plates coated with purified antigens: gp160
(HIV) glycoprotein gp160 is produced in "its native form"
Text from a patent describing how "HIV" proteins (gp160) are produced.
"The envelope gene product of HIV is synthesized as a gp160 precursor molecule,.."
Later on they say:
"..It is therefore most surprising that gp160 may be obtained in its native form."
I would say that if any virus or bacteria is synthesised then we are not dealing with something that belongs to animals, insects, bacteria or viruses.
One way to synthesise a virus is to use a digital camera and take a picture of it. Then you have a visual synthetic version of the virus or the bacteria.
That photo can not attack your blood or immune system.
Or you can create a plastic modell that looks like the animal, bacteria or virus.
The thing is that if we are dealing with anything synthetic that means that it´s not relevant anymore to talk about HIV viruses or stuff taken from it.
Because it's just a chemical version of a creature, the HIV VIRUS! That virus is something they just made up. But they have the "chemical version of it" oh yeah, the synthesised version.
There is no "native HIV".
Once you have synthesised something it means that the thing is dead. As they say in their patent: "..It is therefore most surprising that gp160 (HIV virus protein) may be obtained in its native form."
You can call your antibodies what ever you like. It is antibodies against some chemical they use.
Microwell strip plates coated with purified antigens: gp160
(Back to Bio Rads HIV test GS HIV-1/HIV-2 PLUS O EIA)
Human immunodeficiency virus types 1 and 2 recombinant and synthetic peptides GS HIV-1/HIV-2 PLUS O EIA Biological Principles of the Procedure The Genetic Systems HIV-1/HIV-2 plus O EIA is an immunoassay based on the principle of direct the antibody sandwich technique. Microwell strip plates (the solid phase) are coated with purified antigens: gp160
and p24 recombinant proteins derived from HIV-1
(GS HIV-1/HIV-2 PLUS O EIA)
Method for producing native HIV gp160
What is claimed is:
1. A method for the production of native Human Immunodeficiency Virus gp160 comprising: infecting cells from a HUT78 T-cell line with HTLV-III451 ;
(HIV) glycoprotein gp160 is produced in its native form
Human Immunodeficiency Virus (HIV) glycoprotein gp160 is produced in its native form using a clone of HUT78 cells chronically infected with HTLV-III.sub.451, and grown in serum-free medium.
It is therefore most surprising that gp160 may be obtained in its native form.
The procedure of manufacturing an HIV-test kit and it's components
Assignee: Akzo N.V. (Arnhem, NL) Attorney, Agent or Firm: Blackstone, William M.
Claims: What is claimed is:
1. A method for the production of native human immunodeficiency virus gp160 comprising:
infecting cells from a HUT78 T-cell line with HTLV-III451 ;
selecting infected HUT78 cells that produce native gp160;
incubating said gp160 producing cell line in serum-free medium under conditions that promote cell growth; and
isolating native gp160 from said medium.
2. In a process for the production of envelope proteins from human immunodeficiency virus wherein a T-cell line is infected with said virus, incubated in a medium under conditions that promote cell growth, and glycoproteins are secreted into said medium, the improvement which comprises:
incubating cells from a gp160 producing 6D5451 infected T-cell line in serum-free medium, and
isolating from said medium a 160 kD protein which corresponds to native HIV envelope glycoprotein gp160.
Description: BACKGROUND OF THE INVENTION
The present invention relates to the production of native gp160 of human immunodeficiency virus (HIV), the etiologic agent of Acquired Immune Deficiency Syndrome (AIDS).
Human Immunodeficiency Virus (HIV) is now well established as the etiological agent of acquired immunodeficiency syndrome (AIDS). The virus is tropic for cells bearing the CD4 antigen and is highly cytopathic for helper-inducer (T4) cells. The envelope gene product of HIV is synthesized as a gp160 precursor molecule,
which is subsequently processed into the external envelope protein gp120 and the transmembrane protein gp41. The precursor/product relationship between gp160 and the smaller proteins, gp120 and gp41, has now been well documented, as well as the amino acid sequences of all three [Allan, et al., Science, 228:1091-1094 (1985) and Veronese, et al, Science, 229:1402-1405 (1985)]. The external glycoprotein gp120 binds to the CD4 molecule on susceptible cells in the initial phase of viral cell fusion and giant cell formation induced by the virus [Dalgleish, et al., Nature, 312:763-766].
In addition to their role in cell surface receptor recognition and cell fusion, HIV gp120 and gp41 are the primary targets for immune recognition in individuals infected with HIV. Hence, these proteins have received special attention in virus neutralization studies and vaccine development. It has been observed that large segments of gp120 expressed by recombinant DNA techniques, or native gp120 purified from HIV-infected cells, elicit mostly type-specific neutralizing antibodies in animals. In addition, the HIV envelope precursor protein gp160 expressed in insect cells with baculovirus vectors produced a strong type-specific immune response in goats [Rusche, et al., PNAS, USA 84:6924-6928 (1987)].
The ability to infect certain cell lines with HIV, and to establish the infected cells into a continuous producer of intact virus has been described in U.S. Pat. No. 4,652,599. Even the ability to infect the cell line and the HIV variant of the present invention have been previously described [Getchell, et al., J. Clin. Microbiol. 23:737-742 (1986)].
However, neither of these events alone permitted the establishment of a process capable of producing the HIV glycoprotein gp160 in its native form. Normally, native gp160 breaks down into gp120 and gp41. Consequently, the envelope protein obtained from cell culture media or from lysed virus is gp120 and gp41. It is therefore most surprising that gp160 may be obtained in its native form.
Human Immunodeficiency Virus (HIV) glycoprotein gp160 is produced in its native form using a clone of HUT78 cells chronically infected with HTLV-III451, and grown in serum-free medium.
One more important thing is to understand the hidden force that manipulates all internet social media and telepone communication, electronic text messages etc.
I mean if the things I write about HIV/AIDS is true, how come nobody else really talks about it?
People do. Journalists do. But Facebook, youtube, twitter etc is probably silently censored, mail and friend requests on facebook are deleted before you read it. Accounts on Youtube "suspended" etc.
Illuminati control. They control the whole world now through these tools. It's a tool for their global control.
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